RBRC05861

Information1

Image
BRC No.RBRC05861
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common nameB6-RBGS-002, B6-CAGsu9-DsRed2/Acr3-GFP Tg#RBGS002
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. C57BL/6N background.
Strain descriptionTrangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9).
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]
ReferencesExp. Anim., 59, 105-107 (2010). 20224175

Health Report

Examination Date / Room / Rack2020/02/17Room:3-CRack:B
2019/12/16Room:3-CRack:B
2019/10/21Room:3-CRack:B
2019/08/26Room:3-CRack:B
2019/06/17Room:3-CRack:B
2019/04/15Room:3-CRack:B
2019/02/18Room:3-CRack:B
2018/12/17Room:3-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPmouse proacrosin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Su9mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]UNSu9

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)], Discosoma sp. DsRed2 cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, Jellyfish EGFP cDNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 59, 105-107 (2010).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data