Strain Data Sheet

RBRC05861

Strain Information

Image
BRC No.RBRC05861
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common nameB6-RBGS-002, B6-CAGsu9-DsRed2/Acr3-GFP Tg#RBGS002
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. C57BL/6N background.
Strain descriptionTrangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9).
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]
References
Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo.
Hasuwa H, Muro Y, Ikawa M, Kato N, Tsujimoto Y, Okabe M
Exp. Anim., 59, 105-107 (2010). 20224175

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPmouse proacrosin promoter
Su9mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]UNSu9

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)], Discosoma sp. DsRed2 cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, Jellyfish EGFP cDNA
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 59, 105-107 (2010).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: TBUSAMTA Nonprofit
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data