RBRC05859

Information1

Image
BRC No.RBRC05859
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Pdilt<tm1Osb>/70
Former Common namePdilt KO, Pdilt KO#70
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Masayo Ikawa and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain descriptionPdilt gene knockout mice. Homozygous mutant mice are viable, but males are infertile.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:B6D2F1]
ReferencesProc. Natl. Acad. Sci. USA, 109, 3850-3855 (2012). 22357757

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pdiltprotein disulfide isomerase-like, testis expressed7Pdilt<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7

Information2

Donor DNAmouse Pdilt genomic DNA, E. coli Neomycin resistance gene, yeast FRT (flipase recombination target) sites【クラス1】 (以下「残存の可能性有」を交配にて取り除く)Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal 【クラス1】
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 109, 3850-3855 (2012).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data