Strain Data Sheet

RBRC05856

Strain Information

Image
BRC No.RBRC05856
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Dcst1)Osb
Former Common nameB6D2F1-Tg(Dcst1-mouse Dcst1)<Osb>, DCST1 TG
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Naokazu Inoue, Research Institute for Microbial Diseases, Osaka University in 2012. C57BL/6 and DBA/2 mixed background.
Strain descriptionMouse Dcst1 gene transgenic mice.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6D2F1]Tg mice are viable and fertile.
References
Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion.
Inoue N, Hagihara Y, Wada I
Elife, 10:e66313 (2021). 33871360

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dcst1DC-STAMP domain containing 1UNDcst1

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Dcst1 genomic DNA, pSC-B-amp/kanベクター配列【クラス１】
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Elife. 2021 Apr 19;10:e66313.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorNaokazu Inoue (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data