Strain Data Sheet

RBRC05851

Strain Information

Image
BRC No.RBRC05851
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Flg<tm1Amag>
Former Common nameBALB-FLG KO, BALB-Flg KO, C.Cg-Flg<tm1>
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Dr. Akiharu Kubo and Masayuki Amagai, Keio University School of Medicine in 2008. BA1 ES cells were used. The mutant mice were backcrossed to BALB/c by using speed congenic method (99.4% replacement). C57BL/6J background (RBRC05850), BALB/c (RBRC05851).
Strain descriptionFlg, filaggrin gene knockout mice. The start codon in exon 2 and in-frame ATG sequences of exon 3 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable, but exhibit dry scaly skin.
Colony maintenanceHeterozygote x Homozygote [or Crossing to BALB/cAJcl]
References
Altered stratum corneum barrier and enhanced percutaneous immune responses in filaggrin-null mice.
Kawasaki H, Nagao K, Kubo A, Hata T, Shimizu A, Mizuno H, Yamada T, Amagai M
J. Allergy Clin. Immunol., 129, 1538-1546 (2012). 22409988

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Flgfilaggrin3Flgtargeted mutation 1, Masayuki Amagai
Frtyeast FRT (flippase recombination target) site3Frt
Frtyeast FRT (flippase recombination target) site3Frt
loxPphage P1 loxP3loxP
loxPphage P1 loxP3loxP
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal epidermis stratum corneum morphology(MP:0001240)

  • abnormal epidermis stratum granulosum morphology(MP:0001239)

  • abnormal humoral immune response(MP:0001800)

  • abnormal lipid level(MP:0001547)

  • abnormal skin morphology(MP:0002060)
    • more 14 phenotypes
  • decreased keratohyalin granule number(MP:0009605)

  • dermatitis(MP:0001194)

  • dry skin(MP:0003853)

  • epidermal desquamation(MP:0001230)

  • hyperkeratosis(MP:0001242)

  • increased IgE level(MP:0002497)

  • increased IgG1 level(MP:0008499)

  • increased cholesterol level(MP:0003982)

  • increased inflammatory response(MP:0001846)

  • increased susceptibility to type IV hypersensitivity reaction(MP:0005617)

  • integument phenotype(MP:0010771)

  • scaly skin(MP:0001192)

  • shiny skin(MP:0001196)

  • skin lesions(MP:0001212)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Saccharomyces cerevisiae Frt (Flp recombinase target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), Bacteria transposon Tn5, Neomycin resistance gene, mouse PGK polyA, mouse Flg genomic DNA
    Research applicationCre/loxP system
    Dermatology Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, and shall agree to decide the co-authorship of the DEPOSITOR based agreement with the DEPOSITOR in the case of any publications based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayuki Amagai (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2013

    BRC mice in Publications

    No Data