Strain Data Sheet

RBRC05850

Information1

Image
BRC No.RBRC05850
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Flg<tm1Amag>/Rbrc
Former Common nameB6-FLG KO, B6-Flg KO, B6.Cg-Flg<tm1>
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Akiharu Kubo and Masayuki Amagai, Keio University School of Medicine in 2008. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6J by using speed congenic method (100 makers were replaced to C57BL/6 type). C57BL/6J background (RBRC05850), BALB/c (RBRC05851).
Strain descriptionFlg, filaggrin gene knockout mice. The start codon in exon 2 and in-frame ATG sequences of exon 3 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable, but exhibit dry scaly skin.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Allergy Clin. Immunol., 129, 1538-1546 (2012). 22409988

Health Report

Examination Date / Room / Rack2021/10/26Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Flgfilaggrin3Flg<tm1Amag>targeted mutation 1, Masayuki Amagai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, Saccharomyces cerevisiae Frt (Flp recombinase target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), Bacteria transposon Tn5, Neomycin resistance gene, mouse PGK polyA, mouse Flg genomic DNA
Research applicationDermatology Research
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, and shall agree to decide the co-authorship of the DEPOSITOR based agreement with the DEPOSITOR in the case of any publications based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayuki Amagai (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Sep 2013
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data