Strain Data Sheet

RBRC05850

Strain Information

Image
BRC No.RBRC05850
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Flg<tm1Amag>/Rbrc
Former Common nameB6-FLG KO, B6-Flg KO, B6.Cg-Flg<tm1>
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Akiharu Kubo and Masayuki Amagai, Keio University School of Medicine in 2008. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6J by using speed congenic method (100 makers were replaced to C57BL/6 type). C57BL/6J background (RBRC05850), BALB/c (RBRC05851).
Strain descriptionFlg, filaggrin gene knockout mice. The start codon in exon 2 and in-frame ATG sequences of exon 3 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable, but exhibit dry scaly skin.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Altered stratum corneum barrier and enhanced percutaneous immune responses in filaggrin-null mice.
Kawasaki H, Nagao K, Kubo A, Hata T, Shimizu A, Mizuno H, Yamada T, Amagai M
J. Allergy Clin. Immunol., 129, 1538-1546 (2012). 22409988

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Flg
MGI:95553		filaggrin3Flg<tm1Amag>
MGI:5496587		targeted mutation 1, Masayuki Amagai
Frt yeast FRT (flippase recombination target) site3Frt
Frt yeast FRT (flippase recombination target) site3Frt
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP
neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal epidermis stratum corneum morphology (MP:0001240)

  • abnormal epidermis stratum granulosum morphology (MP:0001239)

  • abnormal humoral immune response (MP:0001800)

  • abnormal lipid level (MP:0001547)

  • abnormal skin morphology (MP:0002060)
    • more 14 phenotypes
  • decreased keratohyalin granule number (MP:0009605)

  • dermatitis (MP:0001194)

  • dry skin (MP:0003853)

  • epidermal desquamation (MP:0001230)

  • hyperkeratosis (MP:0001242)

  • increased IgE level (MP:0002497)

  • increased IgG1 level (MP:0008499)

  • increased cholesterol level (MP:0003982)

  • increased inflammatory response (MP:0001846)

  • increased susceptibility to type IV hypersensitivity reaction (MP:0005617)

  • integument phenotype (MP:0010771)

  • scaly skin (MP:0001192)

  • shiny skin (MP:0001196)

  • skin lesions (MP:0001212)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Saccharomyces cerevisiae Frt (Flp recombinase target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), Bacteria transposon Tn5, Neomycin resistance gene, mouse PGK polyA, mouse Flg genomic DNA
    Research applicationDermatology Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, and shall agree to decide the co-authorship of the DEPOSITOR based agreement with the DEPOSITOR in the case of any publications based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayuki Amagai (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2013

    BRC mice in Publications

    No Data