BRC No.RBRC05847
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn
Former Common nameEdnrb(-/-); DBH-Ednrb; Dct-LacZ+/+, B6-Ednrb KO/DBH-Ednrb/Dct-lacZ
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Strain developmentDeveloped by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. Ddnrb KO and DBH-Ednrb Tg alleles were generated by Masashi Yanagisawa, Howard Hughes Medical Institute. Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.
Strain descriptionEdnrb KO/DBH-Ednrb Tg/Dct-lacZ Tg mice. Ednrb homozygous and DBH-Ednrb Tg mice are viable due to a lethal megacolon phenotype of Ednrb KO mice are rescued by the DBH-Ednrb expression. These mice also express lacZ in melanocytes under the control of Dct promoter.
Colony maintenanceEdnrb KO : Homozygote x Heterozygote ; DBH-Ednrb : Carrier x Carrier ; Dct-lacZ : Carrier x Carrier (Homozygote x Homozygote)
ReferencesCell, 79, 1267-1276 (1994). 8001159
Dev. Biol., 192, 99-107 (1997). 9405100
J. Clin. Invest., 102, 1092-1101 (1998). 9739043
Pigment Cell Res., 19, 644-645 (2006). 17083491

Health Report

Examination Date / Room / Rack2020/06/01Room:3-ARack:I


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ednrbendothelin receptor type B14Ednrb<tm1Ywa>targeted mutation 1, Masashi Yanagisawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ednrbendothelin receptor type B (Rat)UNEdnrbhuman dopamine beta hydroxylase (DBH) promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNmouse dopachrome tautomerase promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 splice and polyadenylation signalsUN


Donor DNAEscherichia coli Neomycin resistance gene, Mus msuculus PGK promoter, Rattus norvegicus Endothelin receptor beta, Homo sapiens DBH-promoter, Escherichia coli LacZ, Mus msuculus Dct promoter, SV40 splice and polyadenylation signals
Research applicationFluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The DEPOSITOR of BIOLOGICAL RESOURCE must obtain a consent on use of it from the DEVELOPER of the Ednrbtm1Ywa and Tg(DBH-Ednrb)Ywa (Professor Masashi Yanagisawa, University of Tsukuba (yanagisawa.masa.fu@u.tsukuba.ac.jp) and the DEVELOPER of Tg(Dct-lacZ)A12Jkn (Professor Ian J.Jackson, Western General Hospital(Edinburgh, UK)(ian.jackson@igmm.ed.ac.uk) before providing the mice to the RECIPIENT.
DepositorMasashi Kato (Chubu University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

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