Strain Data Sheet

RBRC05846

Information1

Image
BRC No.RBRC05846
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Tph2-tTA)1Ahky
Former Common nameTph2-tTA, B6-Tph2-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2010. Tph2 locus of the BAC RP23-112F24 clone was replaced with tTA.
Strain descriptionSerotonin neuron neuron specific tTA BAC transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
ReferencesInt J Neuropsychopharmacol. 17(11):1777-83 (2014). 24834486

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainmouse tryptophan hydroxylase 2 promoter

Information2

Donor DNAE. coli Tet repressor (tetR), HSV VP16 activation domain, SV40 polyA, mouse BAC RP23-112F24 (Phage P1 loxP site, yeast FRT (flipase recombination target) site)
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

N OKUMA, M KANAMARU, R MORIYA, KF TANAKA, S ARATA, J WATANABE, A YOSHIKAWA, M IZUMIZAKI.
Optogenetic Stimulation of 5-HT Neurons in the Median Raphe Nucleus Affects Anxiety and Respiration.
Showa Univ. J. Med. Sci. 31(3) 263-274(2019)