BRC No.RBRC05846
SpeciesMus musculus
Strain nameB6.Cg-Tg(Tph2-tTA)1Ahky
Former Common nameTph2-tTA, B6-Tph2-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2010. Tph2 locus of the BAC RP23-112F24 clone was replaced with tTA.
Strain descriptionSerotonin neuron neuron specific tTA BAC transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
ReferencesInt J Neuropsychopharmacol. 17(11):1777-83 (2014). 24834486

Health Report

Examination Date / Room / Rack2014/01/20Room:4-ARack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse tryptophan hydroxylase 2 promoter


Donor DNAE. coli Tet repressor (tetR), HSV VP16 activation domain, SV40 polyA, mouse BAC RP23-112F24 (Phage P1 loxP site, yeast FRT (flipase recombination target) site)
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data