RBRC05845

Information1

Image
BRC No.RBRC05845
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(hOX-Arch/EGFP)1Ahky
Former Common nameOrexin-Arch, B6-hOX-Arch/GFP Tg
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2009. C57BL/6 and DBA/2 mixed background.
Strain descriptionThese transgenic mice express an Archaerhodopsin/EGFP fusion protein under the control of the Orexin promoter.
Colony maintenanceCarrier x Carrier [or Crossing to Jcl:B6D2F1]Tg homozygous and hemizygous mice are viable and fertile.
ReferencesJ. Neuroendocrinol., 22(7): 818-824 (2010). 20456607
Behav. Brain Res., 255:64-74 (2013). 23707248

Health Report

Examination Date / Room / Rack2013/10/21Room:3-CRack:F
2013/08/26Room:3-CRack:F
2013/07/02Room:3-CRack:F
2013/06/17Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
ArchArchaerhodopsinUNArchhuman orexin (hypocretin) promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFP

Information2

Donor DNAhuman orexin (hypocretin) promoter, Halorubrum Archaerhodopsin, jellyfish GFP cDNA, mouse Protamine I polyA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data