Strain Data Sheet



BRC No.RBRC05845
SpeciesMus musculus
Strain nameB6D2-Tg(hOX-Arch/EGFP)1Ahky
Former Common nameOrexin-Arch, B6-hOX-Arch/GFP Tg
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2009. C57BL/6 and DBA/2 mixed background.
Strain descriptionThese transgenic mice express an Archaerhodopsin/EGFP fusion protein under the control of the Orexin promoter.
Colony maintenanceCarrier x Carrier [or Crossing to Jcl:B6D2F1]Tg homozygous and hemizygous mice are viable and fertile.
ReferencesJ. Neuroendocrinol., 22(7): 818-824 (2010). 20456607
Behav. Brain Res., 255:64-74 (2013). 23707248

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ArchArchaerhodopsinUNArchhuman orexin (hypocretin) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP


Donor DNAhuman orexin (hypocretin) promoter, Halorubrum Archaerhodopsin, jellyfish GFP cDNA, mouse Protamine I polyA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

Williams RH, Tsunematsu T, Thomas AM, Bogyo K, Yamanaka A, Kilduff TS.
Transgenic Archaerhodopsin-3 Expression in Hypocretin/Orexin Neurons Engenders Cellular Dysfunction and Features of Type 2 Narcolepsy.
J Neurosci 39(47) 9435-9452(2019) 31628177