Strain Data Sheet


Strain Information

BRC No.RBRC05844
SpeciesMus musculus
Strain nameC57BL/6-Tg(Pmch-tTA)BAhky
Former Common nameMCH-tTA, B6-MCH-tTA Tg, C57BL/6-Tg(MCH-tTA)1Ahky
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2010. C57BL/6J background. MCH locus of the BAC RP23-348H6 clone was replaced with tTA.
Strain descriptionMelanin-concentrating hormone (MCH) neuron specific tTA BAC transgenic mice.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg mice are viable and fertile.
ReferencesJ. Neurosci., 34(20):6896-909 (2014). 24828644

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainmouse melanin-concentrating hormone (MCH) promoter

Ordering Information

Donor DNAmouse melanin-concentrating hormone (MCH) genomic DNA (mouse BAC PR23-348H6), tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), SV40 polyA, Phage P1 loxP site from BAC vector
Research applicationNeurobiology Research
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data