Strain Data Sheet

RBRC05843

Strain Information

Image
BRC No.RBRC05843
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Actb<tm1(tetO-ChR2*E123T/T159C)Ahky>
Former Common nameTetO ET/TC, B6-tetO-ChR2/ETTC KI
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2012.
Strain descriptionThe tetO-ChR2(E123T/T159C) cassette was inserted into the Actb gene.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]Homozygous mice are viable and fertile.
ReferencesJ. Neurosci. 34, 6896-6909 (2014). 24828644
Proc. Natl. Acad. Sci. USA, 108, 7595-600 (2011). 21504945

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb <tm1(tetO-ChR2*E123T/T159C)Ahky>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin intron5HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA5

Ordering Information

Donor DNAE. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron, C. reinhardtii Channelrhodopsin2, jellyfish YFP cDNA, SV40 polyA, mouse Beta actin, P1 phage loxP sites, yeast FRT (flipase recomnibation target) site, mouse Actb genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Dec 2013

BRC mice in Publications

Suzuki E, Momiyama T.
M1 muscarinic acetylcholine receptor-mediated inhibition of GABA release from striatal medium spiny neurons onto cholinergic interneurons.
Eur J Neurosci 53(3) 796-813(2021) 33270289