Strain Data Sheet

RBRC05842

Information1

Image
BRC No.RBRC05842
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(tetO-ArchT/EGFP)1Ahky
Former Common nameTetO ArchT, B6-tetO-ArchT/GFP Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDr. Akihiro Yamanaka, National Institute for Physiological Sciences in 2011. C57BL/6J background.
Strain descriptionThese transgenic mice express an Archaerhodopsin-T/EGFP fusion protein under the control of the TRE (tetracycline responsive element) promoter.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]Tg homozygous and hemizygous mice are viable and fertile.
ReferencesBehav. Brain Res., 255, 64-74 (2013). 23707248
Front. Syst. Neurosci., 2011;5:18. doi: 10.3389/fnsys.2011.00018. Epub 2011 Apr 13. 21811444

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ArchTArchaerhodopsin-T (Halorubrum strain TP009)UNArchTE. coli Tetracycline operator, CMV minimal promotor, rabbit Beta globin intron

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNAE. coli Tetracycline operator, CMV minimal promotor, rabbit Beta globin intron, Halorubrum strain TP009 Archaerhodopsin, jellyfish GFP cDNA, SV40 polyA, mouse Beta actin, P1 phage loxP sites, yeast FRT (flipase recomnibation target) site
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data