RBRC05821

Information1

Image
BRC No.RBRC05821
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Camk2a<tm1.1Oyam>
Former Common nameB6-CaMKII alpha (K42R), B6-Camk2a KI/K42R
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yoko Yamagata and Yuchio Yanagawa, National Institute for Physiological Sciences in 2003. Derived from TT2 ES cell line (B6CBAF1 derived). The PGK-neo cassette was removed by transient expression of cre recombinase in ES cells. C57BL/6 background (RBRC05821), ICR mixed background (RBRC05636).
Strain descriptionCamk2a/K42R knock-in mice. Ca<2+>/calmodulin-dependent protein kinase IIalpha (CaMKII alpha/ Camk2a) is an enzyme that adds phosphates to a variety of protein substrates to modify their functions. CaMKIIalpha is especially enriched in the hippocampus, and is believed to be an essential mediator of activity-dependent synaptic plasticity and memory functions. We generated a novel kinase-dead knock-in mouse of the CaMKII alpha gene that completely and exclusively lacks its kinase activity by introducing a point mutation (K42R) that prevents ATP binding necessary for phosphorylation reaction. Both heterozygous and homozygous mice are hyperactive, but otherwise indistinguishable from wild-type mice. They are fertile and without any obvious neurological symptoms, except for sporadic observation of mild limbic seizure. Homozygous mice are severely impaired with hippocampal synaptic plasticity and hippocampus-dependent memory formation. Suitable for studies of the mechanisms of LTP and learning and memory, and as a memory-deficit animal model.
Colony maintenanceBack crossed to C57BL/6NCrlCrlj for more than 20 generations.
ReferencesJ. Neurosci., 29, 7607-7618 (2009). 19515929

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Camk2acalcium/calmodulin-dependent protein kinase II alpha18Camk2a<tm1.1Oyam>targeted mutation 1.1, Yoko Yamagata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Information2

Donor DNAP1 Phage loxP site, mouse Camk2a genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 29, 7607-7618 (2009).
DepositorYoko Yamagata (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (more than 6 months)
Additional Info.

BRC mice in Publications

No Data