Strain Data Sheet

RBRC05808

Strain Information

Image
BRC No.RBRC05808
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Aldh2<tm1Kaw>
Former Common nameAldh2KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kyoko Kitagawa and Toshihiro Kawamoto, University of Occupational and Environmental Health in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 7 times.
Strain descriptionAldh2 gene knockout mice. A termination codon and a neomycin selection cassette was inserted into exon 3 of Aldh2 gene.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
References
Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse.
Kitagawa K, Kawamoto T, Kunugita N, Tsukiyama T, Okamoto K, Yoshida A, Nakayama K, Nakayama K
FEBS Lett., 476, 306-311 (2000). 10913633

An essential role for mitochondrial aldehyde dehydrogenase in nitroglycerin bioactivation.
Chen Z, Foster M W, Zhang J, Mao L, Rockman H A, Kawamoto T, Kitagawa K, Nakayama K I, Hess D T, Stamler J S
Proc. Natl. Acad. Sci. USA, 102, 12159-12164 (2005). 16103363

Aldehyde dehydrogenase 2 gene targeting mouse lacking enzyme activity shows high acetaldehyde level in blood, brain, and liver after ethanol gavages.
Isse T, Matsuno K, Oyama T, Kitagawa K, Kawamoto T
Alcohol Clin. Exp. Res., 29, 1959-1964, (2005). 16340452

Characteristics of aldehyde dehydrogenase 2 (Aldh2) knockout mice.
Yu H S, Oyama T, Isse T, Kitakawa K, Ogawa M, Pham T T, Kawamoto T
Toxicol. Mech. Methods., 19(9), 535-540 (2009). 19874182

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aldh2
MGI:99600
aldehyde dehydrogenase 2, mitochondrial5Aldh2<tm1Kaw>
MGI:2157245
targeted mutation 1, Toshihiro Kawamoto
  • alcohol sensitivity, acute(MedGEN)
  • neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA signal5

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cellular respiration(MP:0010954)

  • abnormal enzyme/coenzyme activity(MP:0005584)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 Poly A signal, mouse Aldh2 genomic DNA
    Research applicationMetabolism Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Kasai A, Jee E, Tamura Y, Kouzaki K, Kotani T, Nakazato K.
    Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice.
    Am J Physiol Regul Integr Comp Physiol 322(6) R511-R525(2022) 35318866
    Xu T, Guo J, Wei M, Wang J, Yang K, Pan C, Pang J, Xue L, Yuan Q, Xue M, Zhang J, Sang W, Jiang T, Chen Y, Xu F.
    Aldehyde dehydrogenase 2 protects against acute kidney injury by regulating autophagy via the Beclin-1 pathway.
    JCI Insight 6(15) (2021) 34228649
    Wakabayashi Y, Tamura Y, Kouzaki K, Kikuchi N, Hiranuma K, Menuki K, Tajima T, Yamanaka Y, Sakai A, Nakayama KI, Kawamoto T, Kitagawa K, Nakazato K.
    Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
    Am J Physiol Regul Integr Comp Physiol 318(4) R677-R690(2020) 32048867
    Wei S, Zhang L, Bailu Wang, Zhao Y, Dong Q, Pan C, Li C, He D, Yuan Q, Xu F, Chen Y.
    ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.
    Biochem Biophys Res Commun 512(1) 41-48(2019) 30853183
    Cui S, Xue L, Yang F, Dai S, Han Z, Liu K, Liu B, Yuan Q, Cui Z, Zhang Y, Xu F, Chen Y.
    Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.
    J Am Heart Assoc 7(18) e009111(2018) 30371213