RBRC05808

Information1

Image
BRC No.RBRC05808
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Aldh2<tm1Kaw>
Former Common nameAldh2KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kyoko Kitagawa and Toshihiro Kawamoto, University of Occupational and Environmental Health in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 7 times.
Strain descriptionAldh2 gene knockout mice. A termination codon and a neomycin selection cassette was inserted into exon 3 of Aldh2 gene.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
ReferencesFEBS Lett., 476, 306-311 (2000). 10913633
Alcohol Clin. Exp. Res., 29, 1959-1964, (2005). 16340452
Proc. Natl. Acad. Sci. USA, 102, 12159-12164 (2005). 16103363
Toxicol. Mechanisms and Methods, 19, 535-540 (2009). 19874182

Health Report

Examination Date / Room / Rack2015/11/09Room:4-ARack:G
2015/09/28Room:4-ARack:G
2015/07/27Room:4-ARack:G
2015/05/25Room:4-ARack:G
2015/03/23Room:4-ARack:G
2015/01/19Room:4-ARack:G
2014/11/17Room:4-ARack:G
2014/09/16Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Aldh2aldehyde dehydrogenase 2, mitochondrial5Aldh2<tm1Kaw>targeted mutation 1, Toshihiro Kawamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 Poly A signal, mouse Aldh2 genomic DNA
Research applicationMetabolism Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Sumei Cui, Li Xue, Feihong Yang, Shuai Dai, Ziqi Han, Kai Liu, Baoshan Liu, Qiuhuan Yuan, Zhaoqiang Cui, Yun Zhang, Feng Xu, Yuguo Chen.
Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA4-Dependent CCN1 Secretion.
J Am Heart Assoc 7 e009111.(2018) 30371213