Strain Data Sheet

RBRC05808

Information1

Image
BRC No.RBRC05808
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Aldh2<tm1Kaw>
Former Common nameAldh2KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kyoko Kitagawa and Toshihiro Kawamoto, University of Occupational and Environmental Health in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 7 times.
Strain descriptionAldh2 gene knockout mice. A termination codon and a neomycin selection cassette was inserted into exon 3 of Aldh2 gene.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
ReferencesAlcohol Clin. Exp. Res., 29, 1959-1964, (2005). 16340452
Proc. Natl. Acad. Sci. USA, 102, 12159-12164 (2005). 16103363
Toxicol. Mech. Methods., 19(9), 535-540 (2009). 19874182
FEBS Lett., 476, 306-311 (2000). 10913633

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aldh2aldehyde dehydrogenase 2, mitochondrial5Aldh2<tm1Kaw>targeted mutation 1, Toshihiro Kawamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 Poly A signal, mouse Aldh2 genomic DNA
Research applicationMetabolism Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Kasai A, Jee E, Tamura Y, Kouzaki K, Kotani T, Nakazato K.
Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice.
Am J Physiol Regul Integr Comp Physiol (2022) 35318866

Wakabayashi Y, Tamura Y, Kouzaki K, Kikuchi N, Hiranuma K, Menuki K, Tajima T, Yamanaka Y, Sakai A, Nakayama KI, Kawamoto T, Kitagawa K, Nakazato K.
Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
Am J Physiol Regul Integr Comp Physiol 318(4) R677-R690(2020) 32048867

Wei S, Zhang L, Bailu Wang, Zhao Y, Dong Q, Pan C, Li C, He D, Yuan Q, Xu F, Chen Y.
ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression.
Biochem Biophys Res Commun 512(1) 41-48(2019) 30853183

Cui S, Xue L, Yang F, Dai S, Han Z, Liu K, Liu B, Yuan Q, Cui Z, Zhang Y, Xu F, Chen Y.
Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.
J Am Heart Assoc 7(18) e009111(2018) 30371213

Xu T, Guo J, Wei M, Wang J, Yang K, Pan C, Pang J, Xue L, Yuan QH, Xue M, Zhang J, Sang W, Jiang T, Chen Y, Xu F.
Aldehyde dehydrogenase 2 protects against acute kidney injury by regulating autophagy via Beclin-1 pathway.
JCI Insight (2021) 34228649