Strain Information | |
---|---|
Image | |
BRC No. | RBRC05808 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.129P2-Aldh2<tm1Kaw> |
Former Common name | Aldh2KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Kyoko Kitagawa and Toshihiro Kawamoto, University of Occupational and Environmental Health in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 7 times. |
Strain description | Aldh2 gene knockout mice. A termination codon and a neomycin selection cassette was inserted into exon 3 of Aldh2 gene. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile. |
References | Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse. Kitagawa K, Kawamoto T, Kunugita N, Tsukiyama T, Okamoto K, Yoshida A, Nakayama K, Nakayama K FEBS Lett., 476, 306-311 (2000). 10913633An essential role for mitochondrial aldehyde dehydrogenase in nitroglycerin bioactivation. Chen Z, Foster M W, Zhang J, Mao L, Rockman H A, Kawamoto T, Kitagawa K, Nakayama K I, Hess D T, Stamler J S Proc. Natl. Acad. Sci. USA, 102, 12159-12164 (2005). 16103363Aldehyde dehydrogenase 2 gene targeting mouse lacking enzyme activity shows high acetaldehyde level in blood, brain, and liver after ethanol gavages. Isse T, Matsuno K, Oyama T, Kitagawa K, Kawamoto T Alcohol Clin. Exp. Res., 29, 1959-1964, (2005). 16340452Characteristics of aldehyde dehydrogenase 2 (Aldh2) knockout mice. Yu H S, Oyama T, Isse T, Kitakawa K, Ogawa M, Pham T T, Kawamoto T Toxicol. Mech. Methods., 19(9), 535-540 (2009). 19874182 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Aldh2 MGI:99600 | aldehyde dehydrogenase 2, mitochondrial | 5 | Aldh2<tm1Kaw> MGI:2157245 | targeted mutation 1, Toshihiro Kawamoto | |||
neo | neomycin resistance gene (E. coli) | 5 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
SV40 polyA signal | 5 |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 Poly A signal, mouse Aldh2 genomic DNA |
Research application | Metabolism Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. |
Depositor | Keiichi Nakayama (Kyushu University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
Kasai A, Jee E, Tamura Y, Kouzaki K, Kotani T, Nakazato K. Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice. Am J Physiol Regul Integr Comp Physiol 322(6) R511-R525(2022) 35318866 |
Xu T, Guo J, Wei M, Wang J, Yang K, Pan C, Pang J, Xue L, Yuan Q, Xue M, Zhang J, Sang W, Jiang T, Chen Y, Xu F. Aldehyde dehydrogenase 2 protects against acute kidney injury by regulating autophagy via the Beclin-1 pathway. JCI Insight 6(15) (2021) 34228649 |
Wakabayashi Y, Tamura Y, Kouzaki K, Kikuchi N, Hiranuma K, Menuki K, Tajima T, Yamanaka Y, Sakai A, Nakayama KI, Kawamoto T, Kitagawa K, Nakazato K. Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle. Am J Physiol Regul Integr Comp Physiol 318(4) R677-R690(2020) 32048867 |
Wei S, Zhang L, Bailu Wang, Zhao Y, Dong Q, Pan C, Li C, He D, Yuan Q, Xu F, Chen Y. ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression. Biochem Biophys Res Commun 512(1) 41-48(2019) 30853183 |
Cui S, Xue L, Yang F, Dai S, Han Z, Liu K, Liu B, Yuan Q, Cui Z, Zhang Y, Xu F, Chen Y. Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion. J Am Heart Assoc 7(18) e009111(2018) 30371213 |