RBRC05799

Information1

Image
BRC No.RBRC05799
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rab33a<tm1Mitf>
Former Common nameRab33A KO, B6-Rab33A KO
H-2 Haplotype
ES Cell lineBA1 [(C57BL/6 x 129/SvEv)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Mitsunori Fukuda, Graduate School of Life Sciences, Tohoku University in 2006. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 10 times.
Strain descriptionRab33A gene knockout mice. Exon 1 was replaced with a neo cassette.
Colony maintenanceHomozygote (female) x Hemizygote (male) [or Crossing to C57BL/6]Homozygous mutant mice show no obvious abnormality.
References

Health Report

Examination Date / Room / Rack2013/08/26Room:3-CRack:C
2013/07/10Room:3-CRack:C
2013/07/08Room:3-CRack:C
2013/06/17Room:3-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rab33aRAB33A, member of RAS oncogene familyXRab33a<tm1Mitf>targeted mutation 1, Mitsunori Fukuda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)X

Information2

Donor DNAE. coli Neomycin resistance gene, Phage P1 loxP, Yeast frt sites
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the BIOLOGICAL RESOURCE.
DepositorMitsunori Fukuda (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data