Strain Data Sheet


Strain Information

BRC No.RBRC05796
SpeciesMus musculus
Strain nameSTOCK Tc(HSA21q-MAC1)
Former Common nameDS-MAC, HSA21-MAC Tc
H-2 Haplotype
ES Cell lineTT2F [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Strain developmentDeveloped by Yasuhiro Kazuki, Graduate School of Medical Sciences in 2011. Mixed background. Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile.
Strain descriptionTranschromosomic mice. 21q of the human chromosome was transferred into mouse embryonic stem cell by using mouse artificial chromosome (MAC) vectors Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile.
Colony maintenanceHemizygote (female) x Wild-type (male) [or Crossing to Slc : B6D2F1]Homozygous (2 copies) mutant mice are lethal. Hemizygous mutant males are infertile.
ReferencesElife, 9:e56223 (2020). 32597754
ACS Synth. Biol., (2012). 23654256

Health Report

Examination Date / Room / Rack2024/06/10Room:3-6Rack:C


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HSA21qUNHSA21qCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Ordering Information

Donor DNAThe Long Arm of Human Chromosome 21, HPRT gene, Bovine growth hormone (BGH) polyA signal, chicken beta-globin insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 large T antigen, SV40 early promoter, SV40 Origin (Ori), polyA signal, P1 Phage loxP site, E. coli ampicilin resistance gene, mouse PGK promoter, E. coli neo resistance gene, E. coli Kan resistance gene, E. coli Cm resistance gene, E. coli hyg resistance gene, jellyfish GFP cDNA, mouse chromosome 11 centromere region
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and Conditions1. The use of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research purpose.
2. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
3. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
4. The RECIPIENT must prior inform the DEPOSITOR whether its research plan includes breeding, crossing with other strains and/or modifications of an inserted DNA.
5. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Kazuki Y. et al. elife. 2020 Jun 29;9:e56223. doi: 10.7554/eLife.56223. PMID: 32597754
6. RIKEN BRC should report the DEPOSITOR the RECIPIENT’s name, address and organization once a year.
DepositorYasuhiro Kazuki (Tottori University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Mar 2021

BRC mice in Publications

Kazuki Y, Gao FJ, Li Y, Moyer AJ, Devenney B, Hiramatsu K, Miyagawa-Tomita S, Abe S, Kazuki K, Kajitani N, Uno N, Takehara S, Takiguchi M, Yamakawa M, Hasegawa A, Shimizu R, Matsukura S, Noda N, Ogonuki N, Inoue K, Matoba S, Ogura A, Florea LD, Savonenko A, Xiao M, Wu D, Batista DA, Yang J, Qiu Z, Singh N, Richtsmeier JT, Takeuchi T, Oshimura M, Reeves RH.
A non-mosaic transchromosomic mouse model of down syndrome carrying the long arm of human chromosome 21.
Elife 9 (2020) 32597754