RBRC05780

Information1

Image
BRC No.RBRC05780
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Rufy3<tm1a(EUCOMM)Hmgu>/B08
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rufy3RUN and FYVE domain containing 35Rufy3<tm1a(EUCOMM)Hmgu>targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor5SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5hBactP (human beta actin promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Rufy3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data