Strain Data Sheet

RBRC05770

Information1

Image
BRC No.RBRC05770
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Pvalb-tTA)1Kftnk
Former Common namePV-tTA, B6-Pvalb-tTA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6, 129 and CBA mixed background.
Strain descriptionParvalbumin-tTA BAC transgenic mice. tTA expression is detected in PV positive cells (cerebellar Purkinje cells and reticular thalamic nuclei in brain).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesCell Rep., 36(4):109427(2021). 34320360
Proc. Natl. Acad. Sci. USA, 109(50), 20720-20725 (2012). 23185019

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tg(Pvalb-tTA)1KftnkUNTg(Pvalb-tTA)1Kftnktransgene insertion 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainmouse parvalbumin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA additional signalUN

Information2

Donor DNAmouse Pvalb genomic DNA (BAC clone RP24-450L20), E. coli tet repressor (tetR), herpes simplex virus (HSV)VP16 activation domain, SV40 polyA additional signal, P1 Phage loxP site, yeast FRT (flipase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A., 109(50), 20720-20725 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data