Strain Data Sheet

RBRC05769

Information1

Image
BRC No.RBRC05769
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6(129S6)-Tg(Aif1-tTA)54Kftnk
Former Common nameIba1-tTA, B6-Aif1-tTA Tg#54
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Kenji Tanaka, National Institute for Physiological Sciences. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. C57BL/6 and 129 mixed background.
Strain descriptionIba1-tTA transgenic mice carry a tTA under the control of the modified Iba1 promoter. tTA expression is detected in Iba1 positive macrophage lineage cells (microglia in brain).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesCell Rep., 2, 397-406 (2012). 22854021

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tg(Aif1-tTA)54KftnkUNTg(Aif1-tTA)54Kftnktransgene insertion 54, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainmouse Iba1 promoter

Information2

Donor DNAmouse Iba1 promoter, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, Bovine Growth Hormone polyA additional signal
Research applicationNeurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 2, 397-406 (2012). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the
use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data