Strain Data Sheet

RBRC05765

Strain Information

Image
BRC No.RBRC05765
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>
Former Common nameH2AZ1/2 floxed, B6-H2AZ1/2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed.
Strain descriptionH2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development.
Colony maintenanceH2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Histone variant H2A.Z is required for early mammalian development.
Faast R, Thonglairoam V, Schulz T C, Beall J, Wells J R, Taylor H, Matthaei K, Rathjen P D, Tremethick D J, Lyons I
Curr. Biol., 11, 1183-1187 (2001). 11516949

Health Report

Examination Date / Room / Rack2025/10/20Room:4-BRack:ESentinel mouse program
2025/08/04Room:4-BRack:ESentinel mouse program
2025/05/26Room:4-BRack:ESentinel mouse program
2025/02/25Room:4-BRack:ESentinel mouse program
2024/11/25Room:4-BRack:ESentinel mouse program
2024/08/26Room:4-BRack:ESentinel mouse program
2024/05/27Room:4-BRack:ESentinel mouse program
2024/02/26Room:4-BRack:ESentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
H2az2
MGI:1924855		H2A.Z histone variant 211H2az2<tm1.1Hko>
MGI:6163638		targeted mutation 1.1, Haruhiko Koseki
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
Frt yeast FRT (flippase recombination target) site3Frt
H2afz
MGI:1888388		H2A.Z variant histone 13H2afz<tm1.1Hko>
MGI:6163639		targeted mutation 1.1, Haruhiko Koseki
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • embryonic lethality (MP:0008762)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse H2az1 genomic DNA, mouse H2az2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Liang W, Ding J, Chai Q, Lv M, Zheng S, Cao X, Wang Z, Ying X, Wu W, Li G, Zhu M.
    H2A.Z primes an epigenetic landscape for memory CD8+ T cell recall response.
    Nat Commun 16(1) 7625(2025) 40817373
    Reda A, Hategan LA, McLean TAB, Creighton SD, Luo JQ, Chen SES, Hua S, Winston S, Reeves I, Padmanabhan A, Dahi TA, Ramzan F, Brimble MA, Murphy PJ, Walters BJ, Stefanelli G, Zovkic IB.
    Role of the histone variant H2A.Z.1 in memory, transcription, and alternative splicing is mediated by lysine modification.
    Neuropsychopharmacology 49(8) 1285-1295(2024) 38366138
    Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, Senatore A, Monks DA, Liu BH, Leary SC, Cheng HM.
    Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.
    Cell Rep 36(11) 109704(2021) 34525369
    Rispal J, Baron L, Beaulieu JF, Chevillard-Briet M, Trouche D, Escaffit F.
    The H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis.
    Nat Commun 10(1) 1827(2019) 31015444
    Shen T, Ji F, Wang Y, Lei X, Zhang D, Jiao J.
    Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder.
    Nucleic Acids Res 46(5) 2290-2307(2018) 29294103