RBRC05765

Information1

Image
BRC No.RBRC05765
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>
Former Common nameH2AZ1/2 floxed, B6-H2AZ1/2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed.
Strain descriptionH2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development.
Colony maintenanceH2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesCurr. Biol., 11, 1183-1187 (2001). 11516949

Health Report

Examination Date / Room / Rack2018/04/02Room:3-5Rack:C
2018/02/05Room:3-5Rack:C
2017/12/04Room:3-5Rack:C
2017/10/02Room:3-5Rack:C
2017/08/21Room:3-5Rack:C
2017/06/05Room:3-5Rack:C
2017/04/03Room:3-5Rack:C
2017/02/06Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
H2az2H2A.Z histone variant 211H2az2<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
H2afzH2A.Z variant histone 13H2afz<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Information2

Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse H2az1 genomic DNA, mouse H2az2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data