Strain Data Sheet
RBRC05763
Strain Information | |
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| Image | |
| BRC No. | RBRC05763 |
| Type | Targeted Mutation and Transgene |
| Species | Mus musculus |
| Strain name | STOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb |
| Former Common name | Izumo1 KO/Clgn-Izumo1mCherry Tg/CAGAcr-GFP Tg |
| H-2 Haplotype | |
| ES Cell line | D3 [129S2/SvPas] |
| Background strain | Jcl:B6D2F1 |
| Appearance | |
| Strain development | Developed by Dr. Naokazu Inoue and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. C57BL/6 and DBA/2 mixed background. |
| Strain description | Izumo1 knockout and Clgn-Izumo1/mCherry transgene double mutant mice. Izumo1 KO homozygous and Tg positive mice are fertile. The CAG/Acr-GFP transgene is also included. |
| Colony maintenance | KO : Homozygote x Homozygote ; Clgn-Izumo1/mCherry : Carrier x Noncarrier ; CAG/Acr-GFP : Carrier x Carrier [or Crossing to Jcl:B6D2F1] |
| References | Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1. Satouh Y, Inoue N, Ikawa M, Okabe M J. Cell Sci., 125(Pt 21):4985-4990 (2012). 22946049 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Izumo1 MGI:1920706 | izumo sperm-egg fusion 1 | 7 | Izumo1 <tm1Osb> MGI:3576382 | targeted mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
| loxP | phage P1 loxP | 7 | loxP | ||||
| loxP | phage P1 loxP | 7 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 7 | human PGK promoter, human PGK poly (A) | ||||
| GFP | Green Fluorescent Protein (Aequorea victoria) | UN | CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide | ||||
| Izumo1 | izumo sperm-egg fusion 1 | UN | Izumo1 | mouse Clgn promoter | |||
| loxP | phage P1 loxP | UN | loxP | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | UN | mCherry | ||||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Izumo1 genomic DNA, mouse Calmegin promoter, mouse Izumo1 cDNA, coral mCherry cDNA, rabbit beta-globin polyA, bovine growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide, Jellyfish GFP cDNA |
| Research application | Cell Biology Research Cre/loxP system Dermatology Research Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Naokazu Inoue (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |