Strain Data Sheet

RBRC05763

Strain Information

Image
BRC No.RBRC05763
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameIzumo1 KO/Clgn-Izumo1mCherry Tg/CAGAcr-GFP Tg
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Naokazu Inoue and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo1 knockout and Clgn-Izumo1/mCherry transgene double mutant mice. Izumo1 KO homozygous and Tg positive mice are fertile. The CAG/Acr-GFP transgene is also included.
Colony maintenanceKO : Homozygote x Homozygote ; Clgn-Izumo1/mCherry : Carrier x Noncarrier ; CAG/Acr-GFP : Carrier x Carrier [or Crossing to Jcl:B6D2F1]
References
Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1.
Satouh Y, Inoue N, Ikawa M, Okabe M
J. Cell Sci., 125(Pt 21):4985-4990 (2012). 22946049

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Izumo1izumo sperm-egg fusion 17Izumo1 targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
loxPphage P1 loxP7loxP
loxPphage P1 loxP7loxP
neoneomycin resistance gene (E. coli)7human PGK promoter, human PGK poly (A)
GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide
Izumo1izumo sperm-egg fusion 1UNIzumo1mouse Clgn promoter
loxPphage P1 loxPUNloxP
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Izumo1 genomic DNA, mouse Calmegin promoter, mouse Izumo1 cDNA, coral mCherry cDNA, rabbit beta-globin polyA, bovine growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide, Jellyfish GFP cDNA
    Research applicationCell Biology Research
    Cre/loxP system
    Dermatology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorNaokazu Inoue (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data