Strain Data Sheet

RBRC05763

Information1

Image
BRC No.RBRC05763
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameIzumo1 KO/Clgn-Izumo1mCherry Tg/CAGAcr-GFP Tg
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Naokazu Inoue and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo1 knockout and Clgn-Izumo1/mCherry transgene double mutant mice. Izumo1 KO homozygous and Tg positive mice are fertile. The CAG/Acr-GFP transgene is also included.
Colony maintenanceKO : Homozygote x Homozygote ; Clgn-Izumo1/mCherry : Carrier x Noncarrier ; CAG/Acr-GFP : Carrier x Carrier [or Crossing to Jcl:B6D2F1]
ReferencesJ. Cell Sci., 125(Pt 21):4985-4990 (2012). 22946049

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Izumo1izumo sperm-egg fusion 17Izumo1 <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;human PGK promotor, human PGK poly (A)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Izumo1izumo sperm-egg fusion 1UNIzumo1mouse Clgn promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Information2

Donor DNAphage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Izumo1 genomic DNA, mouse Calmegin promotor, mouse Izumo1 cDNA, coral mCherry cDNA, rabbit beta-globin polyA, bovine growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide, Jellyfish GFP cDNA
Research applicationCell Biology Research
Cre/loxP system
Dermatology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorNaokazu Inoue (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data