RBRC05763

Information1

Image
BRC No.RBRC05763
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameIzumo1 KO/Clgn-Izumo1mCherry Tg/CAGAcr-GFP Tg
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Naokazu Inoue and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo1 knockout and Clgn-Izumo1/mCherry transgene double mutant mice. Izumo1 KO homozygous and Tg positive mice are fertile. The CAG/Acr-GFP transgene is also included.
Colony maintenanceKO : Homozygote x Homozygote ; Clgn-Izumo1/mCherry : Carrier x Noncarrier ; CAG/Acr-GFP : Carrier x Carrier [or Crossing to Jcl:B6D2F1]
ReferencesJ. Cell Sci., 125(Pt 21):4985-4990 (2012). 22946049

Health Report

Examination Date / Room / Rack2020/02/17Room:3-ARack:B
2019/12/16Room:3-ARack:B
2019/10/21Room:3-ARack:B
2019/08/26Room:3-ARack:B
2019/06/17Room:3-ARack:B
2019/04/15Room:3-ARack:B
2019/02/18Room:3-ARack:B
2019/01/28Room:3-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Izumo1izumo sperm-egg fusion 17Izumo1 <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7human PGK promotor, human PGK poly (A)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Izumo1izumo sperm-egg fusion 1UNIzumo1mouse calmegin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Information2

Donor DNAmouse Izumo1 cDNA, coral mCherry cDNA, mouse Calmegin promotor, rabbit beta-globin poly (A), bovine growth hormone polyadenylation signal, mouse Izumo1 genomic DNA, E. coli Neomycin resistance gene, yeast FRT (flippase recombination target) sites, Phage P1 loxP, human PGK promotor, human PGK poly (A), Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide [クラス1]
Research applicationCell Biology Research
Cre/loxP system
Dermatology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorNaokazu Inoue (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data