RBRC05761

Information1

Image
BRC No.RBRC05761
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rftn1<tm1Ayos>
Former Common nameRaftlin KO, B6-Rftn1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionRaftlin (Rftn1) gene knockout mice. Exon 2 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit impaired T cell-mediated immune responses.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
ReferencesJ. Immunol., 182, 5929-5937 (2009). 19414744

Health Report

Examination Date / Room / Rack2014/05/19Room:4-ARack:C
2014/03/24Room:4-ARack:C
2014/01/20Room:4-ARack:C
2013/12/04Room:4-ARack:C
2013/11/25Room:4-ARack:C
2013/11/25Room:4-ARack:C
2013/11/25Room:4-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rftn1raftlin lipid raft linker 117Rftn1<tm1Ayos>targeted mutation 1, Akihiko Yoshimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Rftn1 genomic DNA, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene
Research applicationCell Biology Research
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 182, 5929-5937 (2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorAkihiko Yoshimura (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data