RBRC05760

Information1

Image
BRC No.RBRC05760
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spred1<tm1.1Ayos>
Former Common nameSpred1 floxed, B6-Spred1 floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Keio University School of Medicine. A neo cassette flanked by FRT sites was removed by Flp-mediated recombination.
Strain descriptionSpred1 gene floxed mice. Exon 7 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesGenes Cells., 16, 803-814 (2011). 21668589

Health Report

Examination Date / Room / Rack2014/03/10Room:2-検Rack:L
2014/01/14Room:2-検Rack:L
2013/11/11Room:2-検Rack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spred1sprouty protein with EVH-1 domain 1, related sequence2Spred1<tm1.1Ayos>targeted mutation 1.1, Akihiko Yoshimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Information2

Donor DNABacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Spred1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 16, 803-814 (2011).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorAkihiko Yoshimura (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data