RBRC05759

Information1

Image
BRC No.RBRC05759
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spred2<tm1Taga>
Former Common nameSpred2 KO, B6-Spred2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionSpred2 gene knockout mice. Exon 6 was deleted by homologous recombination.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Exp. Med., 199, 737-742 (2004). 14981116

Health Report

Examination Date / Room / Rack2014/09/08Room:2-検Rack:E
2014/07/07Room:2-検Rack:E
2014/05/12Room:2-検Rack:E
2014/03/10Room:2-検Rack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spred2sprouty-related, EVH1 domain containing 211Spred2<tm1Taga>targeted mutation 1, Tetsuya Taga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse Spred2 genomic DNA
Research applicationCell Biology Research
Hematological Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 199, 737-742 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorAkihiko Yoshimura (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data