RBRC05757

Information1

Image
BRC No.RBRC05757
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spry4<tm1Ayos>
Former Common nameSprouty4 KO, B6-Spry4 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University.
Strain descriptionSprouty4 (Spry4) gene knockout mice. Exon 2 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit abnormal mandible morphology. Although a significant fraction of homozygotes die shortly after birth due to mandible defects, the remainder are viable and fertile, but postnatal growth is reduced for most homozygous mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 352, 896-902 (2007). 17156747

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spry4sprouty homolog 4 (Drosophila)18Spry4<tm1Ayos>targeted mutation 1, Akihiko Yoshimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Spry4 genomic DNA, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene
Research applicationDevelopmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophy.s Res. Commun., 352, 896-902 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorAkihiko Yoshimura (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data