Strain Data Sheet

RBRC05754

Information1

Image
BRC No.RBRC05754
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Map3k6<tm1Hijo>
Former Common nameASK2 KO, B6-Map3k6 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2009. E14 ES cells were used. The mutant mice are backcrossed to C57BL/6J.
Strain descriptionMap3k6 gene knockout mice. The exons 1 to 5 were replaced with a NLS-lacZ cassette and a pgk-neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
ReferencesJ. Biol. Chem., 282, 7522-75231 (2007). 17210579
EMBO J., 28, 843-853 (2009). 19214184

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k6mitogen-activated protein kinase kinase kinase 64Map3k6<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)4nls

Information2

Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), Mouse Map3k6 genomic DNA, E. coli beta-galactosidase, SV40 nuclear localization signal sequence, Bovine PolyA signal
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data