Strain Data Sheet

RBRC05753

Information1

Image
BRC No.RBRC05753
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Map3k5<tm1Hijo>
Former Common nameASK1 KO, B6-Map3k5 KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School, Tokyo Medical and Dental University in 2001. J1 ES cells were used. The mutant mice are backcrossed to C57BL/6J.
Strain descriptionMap3k5 gene knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
ReferencesScience., 275, 90-94 (1997). 8974401
EMBO Rep., 2, 222-228 (2001). 11266364

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:H
2023/02/20Room:4-BRack:H
2022/11/29Room:4-BRack:H
2022/08/29Room:4-BRack:H
2022/05/31Room:4-BRack:H
2022/03/07Room:4-BRack:H
2021/12/06Room:4-BRack:H
2021/09/08Room:4-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k5mitogen-activated protein kinase kinase kinase 510Map3k5<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)10GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Phosphoglycerate kinase promoter (Pgk1 promoter), E. coli Neomycin resistance gene, mouse Pgk1 polyA signal, Jellyfish (Aequorea victoria) GFP cDNA, Bovine PolyA signal, Mouse Map3k5 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Aug 2014
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data