RBRC05749

Information1

Image
BRC No.RBRC05749
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(AS071*dSINE-lacZ)E
Former Common nameAS071 delta SINE Tg line E, B6-AS071/dSINE-lacZ#E
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Norihiro Okada, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology in 2012.The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. Three lines were generated : line D (RBRC05748) (main line), line E (RBRC05749), line F (RBRC05750).
Strain descriptionAS071 (dSINE) -lacZ transgenic mice lacking SINE. AS071, one AmnSINE1 locus is a distal enhancer for Fgf8 expressed in the developing diencephalon. The expression pattern of lacZ driven by AS071 partially resembles that of diencephalic Fgf8 (dorsal midline and lateral wall and ventral).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
ReferencesPLoS One, 7, e43785 (2012). 22937095

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNmouse Heat Shock Protein 68 promoter

Information2

Donor DNAMouse Heat Shock Protein 68 promoter, E. coli lacZ gene, SV40 PolyA signal, E. coli Ampicilin resistance gene, Mouse AS071配列 (Chr19 : 45, 641, 837-45, 642, 422, NCBI37/mm9) からSINEに由来する配列203bp (45, 642, 103-45, 642, 305) を除いた配列
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e43785 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorNorihiro Okada (Tokyo Institute of Technology)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data