RBRC05743

Information1

Image
BRC No.RBRC05743
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pknox1<tm1.1Rygo>
Former Common namepKnox1 floxed, B6-Pknox1 floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Dr. Ryo Goitsuka, Tokyo University of Science, Research Institute for Biomedical Sciences in 2008. Bruce 4 ES cells were used. The neo cassette was removed by crossing with FLP mice (B6). The mutant mice were crossed to C57BL/6 over 12 times.
Strain descriptionpKnox1 (Pknox1) gene floxed mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pknox1Pbx/knotted 1 homeobox17Pknox1 <tm1Rygo>targeted mutation 1, Ryo Goitsuka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Information2

Donor DNASaccharomyces cerevisiae FRT sequence, lambda bacteriophage P1 loxP sequence
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorRyo Goitsuka (Tokyo University of Science)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data