RBRC05739

Information1

Image
BRC No.RBRC05739
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Fbxo17<Gt(Ayu21-T57*SACre)1Card>
Former Common nameFbxo17-SACre, B6-bxo17/Cre Gt
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University.
Strain descriptionFbxo17-cre diver mice. The Beta-geo gene in the exchangeable gene trap clones of Fbxo17 gene was replaced with a cre recombinase gene. Ubiquitous X-gal staining was observed in the pups from crossing Fbxo17-cre mice with ROSA26-lacZ mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fbxo17F-box protein 177Fbxo17<tm1(Ayu21-T57*SACre)Imeg>gene trap 1, Center for Animal Resources & Development

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)7En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pgk1mouse PGK polyA signal7Pgk1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase7cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)7nls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)7puromouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, S.alboniger puromycin resistance gene, Phage P1 Cre recombinase, SV40 nuclear localization signal, yeast FRT (flipase recombination target) site, Phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasatake Araki (Kumamoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data