Strain Data Sheet

RBRC05737

Information1

Image
BRC No.RBRC05737
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-tdKaede)15Utr
Former Common nameWK-m15
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Dr. Atsushi Miyawaki, RIKEN Brain Science Institute. C57BL/6 background (RBRC05737), albino C57BL/6 background (RBRC05738).
Strain descriptionThese transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.
Colony maintenanceCarrier x Carrier [Homozygote x Homozygote]
ReferencesProc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008). 18663225
J. Exp. Zool., 216, 113-119 (1981). 6793688

Health Report

Examination Date / Room / Rack2023/07/10Room:3-ARack:C
2023/04/10Room:3-ARack:C
2023/01/10Room:3-ARack:C
2022/10/11Room:3-ARack:C
2022/07/11Room:3-ARack:C
2022/06/06Room:3-ARack:C
2022/04/11Room:3-ARack:C
2022/01/24Room:3-ARack:C
2021/10/26Room:3-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdKaedeTrachyphyllia geoffroy Kaede cDNAUNtdKaedeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA siteUN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Kaede using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
DepositorYoshihiro Miwa (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Aug 2016
Mouse of the Month Dec 2020
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Approval form (form V) from the developer (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Nakamura YK, Janowitz C, Metea C, Asquith M, Karstens L, Rosenbaum JT, Lin P.
Short chain fatty acids ameliorate immune-mediated uveitis partially by altering migration of lymphocytes from the intestine.
Sci Rep 7(1) 11745(2017) 28924192