RBRC05727

Information1

Image
BRC No.RBRC05727
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Syngr4<tm1Osb>
Former Common nameSyngr4 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takao Fujimoto and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2011.
Strain descriptionSyngr4 gene knockout mice. Exon 2 of Syngr4 was replaced with a floxed neo cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6NJcl]
ReferencesProc. Natl. Acad. Sci. USA, 113, 7704-7710 (2016). 27357688

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Syngr4synaptogyrin 47Syngr4 <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Syngr4 genomic DNA, E. coli Neomycin resistance gene, yeast FRT (flippase recombination target) sites, Phage P1 loxP, mouse phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, polyadenylation signal (synthetic)
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A., 113, 7704-7710 (2016).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data