RBRC05722

Information1

Image
BRC No.RBRC05722
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Wbp2nl<tm1a(EUCOMM)Osb>
Former Common namePAWP floxed
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Yuhkoh Satouh and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2011.
Strain descriptionWbp2nl floxed mice. Exons 2 to 4 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [C57BL/6NJcl]
ReferencesBiol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16. 26377222

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Wbp2nlWBP2 N-terminal like15Wbp2nl<tm1a(EUCOMM)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)15En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)15

Information2

Donor DNAEn2 splice acceptor (mouse En2 intron 2 / exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E. coli neomycin phosphotransferase (neoR) gene, yeast FRT (flippase recombination target) sites, phage P1 loxP, mouse Wbp2nl genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. These strains were established by the DEPOSITOR by using knockout vectors of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the vectors of the strains. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data