RBRC05717

Information1

Image
BRC No.RBRC05717
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Pmis2<tm1Osb> Tg(Clgn-Spot3Myc)54Osb
Former Common namePmis2-Myc Tg
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005.
Strain descriptionTestis specific Pmis2 (Myc-tag) gene transgenic mice. Endogenous Pmis2 expression was absent. Tg males are viable.
Colony maintenanceKO : Homozygote x Homozygote ; Tg : Carrier x Carrier [or Crossing to Jcl:B6D2F1]
ReferencesMol. Biol. Cell, 23, 2671-2679 (2012). 22621904

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
4930479M11RikRIKEN cDNA 4930479M11 gene74930479M11Rik <tm1Oka>targeted mutation 1, Masaru Okabe

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
4930479M11RikRIKEN cDNA 4930479M11 geneUN4930479M11Rik

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
myc-tagmyc-epitope tagUNmyc-tag

Information2

Donor DNAmouse Pmis2 genomic DNA, E. coli Neomycin resistance gene, mouse Calmgin promoter, human Myc tag, mouse Pmis2 cDNA, rabbit polyA signal(以下残存の可能性有)Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell, 23, 2671-2679 (2012).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data