Strain Data Sheet

RBRC05704

Information1

Image
BRC No.RBRC05704
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(3xARE/TK-NRF2/luc)M2Tiw
Former Common nameOKD48-LUC, B6-ARE-NRF2/Luc Tg#M2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2010. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J.
Strain descriptionKeap1-dependent Oxidative stress Detector transgenic mice, named OKD48 (Keap1-dependent Oxidative stress Detector, No-48). The mice carry human Nrf2 and Flag-tagged luciferase fusion gene under control of 3xARE promoter (oxidative stress inducible promoter). Only cells experiencing oxidative stress can be detected by luciferase luminescence.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesSci. Rep., 2:229 (2012). 22355743

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
NFE2l2nuclear factor, erythroid derived 2, like 2 (human)UNNFE2l23xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lucluciferase (firefly)UNluc

Information2

Donor DNA3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter), human Nrf2 cDNA, firefly luciferase cDNA, FLAG tag
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 2:229 (2012). The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Suzuki T, Muramatsu A, Saito R, Iso T, Shibata T, Kuwata K, Kawaguchi SI, Iwawaki T, Adachi S, Suda H, Morita M, Uchida K, Baird L, Yamamoto M.
Molecular Mechanism of Cellular Oxidative Stress Sensing by Keap1.
Cell Rep 28(3) 746-758.e4(2019) 31315052