RBRC05704
Information1 | |
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| Image | |
| BRC No. | RBRC05704 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(3xARE/TK-NRF2/luc)M2Tiw |
| Former Common name | OKD48-LUC, B6-ARE-NRF2/Luc Tg#M2 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Takao Iwawaki, Advanced Science Institute, RIKEN in 2010. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J. |
| Strain description | Keap1-dependent Oxidative stress Detector transgenic mice, named OKD48 (Keap1-dependent Oxidative stress Detector, No-48). The mice carry human Nrf2 and Flag-tagged luciferase fusion gene under control of 3xARE promoter (oxidative stress inducible promoter). Only cells experiencing oxidative stress can be detected by luciferase luminescence. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
| References | Sci. Rep., 2:229 (2012). 22355743 |
Health Report | |
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| Examination Date / Room / Rack | 2015/08/05Room:4-ARack:C 2015/08/03Room:4-ARack:C 2015/07/27Room:4-ARack:C 2015/05/25Room:4-ARack:C 2015/03/23Room:4-ARack:C 2015/02/18Room:4-ARack:C 2015/02/16Room:4-ARack:C 2015/01/19Room:4-ARack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele namePromoter FLAGFLAG tag (synthetic)UNFLAG Gene symbolGene nameChr.Allele symbolAllele namePromoter NFE2l2nuclear factor, erythroid derived 2, like 2 (human)UNNFE2l23xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter) Gene symbolGene nameChr.Allele symbolAllele namePromoter lucluciferase (firefly)UNluc |
Information2 | |
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| Donor DNA | 3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter), human Nrf2 cDNA, firefly luciferase cDNA, FLAG tag |
| Research application | Cell Biology Research Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 2:229 (2012). |
| Depositor | Takao Iwawaki (Gunma University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |