RBRC05703

Information1

Image
BRC No.RBRC05703
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Gt(ROSA)26Sor<tm1.1Tiw>
Former Common nameROSA-ERAI-LUCdNeo, B6-ROSA26/ERAI-LUCdNeo
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2006. RF8 ES cells derived from 129S4/SvJae were used. The neo cassette was removed by crossing with Gt(ROSA)26Sor<tm1(FLP1)Dym> mice.
Strain descriptionERAI (ER stress-activated indicator) knock-in mice. Floxed STOP cassette and modified ERAI construct (HA-2xXBP1deltaDBD(anATG)-LUC-F) were inserted into the Gt(ROSA)26Sor locus. The ERAI expression can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesBiochem. J., 425, 117-125 (2009). 19788412

Health Report

Examination Date / Room / Rack2014/07/14Room:4-ARack:E
2014/05/19Room:4-ARack:E
2014/03/24Room:4-ARack:E
2014/01/20Room:4-ARack:E
2013/11/25Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1Tiw>targeted mutation 1.1, Takao Iwawaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
XBP1X-box binding protein 16XBP1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lucluciferase (firefly)6luc

Information2

Donor DNAadenoviral splice acceptor sequences, Bacteriophage P1 loxP sequence, Yeast FRT (flippase recombination target) site, transcriptional STOP cassette (C-terminal sequence of yeast His3 gene, an SV40 polyA and a 54 splice donor site), influenzavirus HA tag, human XBP1 cDNA, firefly luciferase, FLAG tag, SV40 intron and polyA signal
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. J. 425, 117-125 (2010).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jan 2013

BRC mice in Publications

No Data