RBRC05701

Information1

Image
BRC No.RBRC05701
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(FLEXCAG-VSFP-Butterfly)552Bsi
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Thomas Knoepfel, RIKEN Brain Science Institute.
Strain descriptionMice carry the gene for the voltage sensor, VSFP-Butterfly flanked by loxP sites. Expression will be activate by cre recombinase following crossing with a cre driver mouse.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
ReferencesJ. Neurophysiol., 108, 2323-2337 (2012). 22815406
Nat. Rev. Neurosci., 13, 687-700 (2012). 22931891

Health Report

Examination Date / Room / Rack2014/01/20Room:4-ARack:I
2013/11/25Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ci-VSPCiona intestinalis voltage sensor-containing phosphataseUNCi-VSP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory elementUNWPRE

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequenceUNlox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequenceUNlox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCitrinevariant of Yellow Fluorescent ProteinUNmCitrine

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mKateCiona intestinalis, Far-red Fluorescent Protein (sea anemone Entacmaea quadricolor)UNmKateCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNAAequorea victoria Green Fluorescent Protein and derivatives coding sequence, Ciona intestinalis Ci-VSP, Entacmaea quadricolor Far-red Fluorescent Protein (mKate) and derivatives coding sequence, Bovine growth hormone polyadenylation (poly-A) signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorThomas Knöepfel (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Contact Information

BRC mice in Publications

No Data