Strain Data Sheet

RBRC05698

Information1

Image
BRC No.RBRC05698
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-S100a4<tm1>
Former Common names100A4 flox, B6-S100a4 floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Takaji Matsutani and Norihiro Nishimoto in 2009. RENKA ES cells derived from C57BL/6NCrlCrlj were used.
Strain descriptionS100A4 gene floxed mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
S100a4S100 calcium binding protein A43S100a4 <tm1>targeted mutation 1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence3lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Klebsiella pneumoniae)3neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEnterobacteria phage P1 LoxP sites, Saccharomyces cerevisiae FRT sites, mouse phosphoglycerate kinase promoter (PGK promoter), Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycine resistance gene, mouse S100a4 genomic DNA
Research applicationCancer Research
Cell Biology Research
Cre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNorihito Nishimoto (Wakayama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data