Strain Data Sheet
RBRC05691
Information1 | |
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| Image | |
| BRC No. | RBRC05691 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129-Fgf18<tm1Tri> |
| Former Common name | Fgf18 floxed, B6-Fgf18 floxed |
| H-2 Haplotype | |
| ES Cell line | C6 [129/SvEvTac] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Toru Imamura, National Institute of Advanced Industrial Science and Technology in 2009. A FRT-flanked PGK-neo cassette with a 5’loxP site was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 3. A PGK-neo cassette was removed by crossing with FLPe mice (B6-Tg(CAG-FLPe)36 RBRC01834). |
| Strain description | Fgf18 gene floxed mice. Exon 3 of Fgf18 gene was flanked by loxP sites. Conditional Fgf18 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Sometimes curly hairs. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6] |
| References | J. Invest. Dermatol., 132, 1338-1345 (2012). 22297635 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP |
Information2 | |
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| Donor DNA | Bacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Fgf18 genomic DNA |
| Research application | Cre/loxP system Dermatology Research Developmental Biology Research FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol., 132, 1338-1345 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Yoshihiro Ohmiya (National Institute of Advanced Industrial Science and Technology) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |