RBRC05691

Information1

Image
BRC No.RBRC05691
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Fgf18<tm1Tri>
Former Common nameFgf18 floxed, B6-Fgf18 floxed
H-2 Haplotype
ES Cell lineC6 [129/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Toru Imamura, National Institute of Advanced Industrial Science and Technology in 2009. A FRT-flanked PGK-neo cassette with a 5’loxP site was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 3. A PGK-neo cassette was removed by crossing with FLPe mice (B6-Tg(CAG-FLPe)36 RBRC01834).
Strain descriptionFgf18 gene floxed mice. Exon 3 of Fgf18 gene was flanked by loxP sites. Conditional Fgf18 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Sometimes curly hairs.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6]
ReferencesJ. Invest. Dermatol., 132, 1338-1345 (2012). 22297635

Health Report

Examination Date / Room / Rack2013/11/11Room:2-検Rack:B
2013/09/09Room:2-検Rack:B
2013/07/08Room:2-検Rack:B
2013/05/13Room:2-検Rack:B
2013/03/04Room:2-検Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fgf18fibroblast growth factor 1811Fgf18<tm1Tri>targeted mutation 1, Toru Imamura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Information2

Donor DNABacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Fgf18 genomic DNA
Research applicationCre/loxP system
Dermatology Research
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol., 132, 1338-1345 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoshihiro Ohmiya (National Institute of Advanced Industrial Science and Technology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data