Strain Data Sheet

RBRC05690

Information1

Image
BRC No.RBRC05690
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sst<tm1a(KOMP)Wtsi>/F04
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sstsomatostatin16Sst<tm1a(KOMP)Wtsi>targeted mutation 1a, Wellcome Trust Sanger Institute

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal16

Information2

Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Sst genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data