BRC No.RBRC05665
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rasgrp4<tm1.2>
Former Common nameRasGRP4 KO mouse, B6-RasGRP4 KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2007. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6). And then, Exons 5 and 6 were deleted by crossing with CAG-cre mice.
Strain descriptionRasGRP4 (Rasgrp4) gene knockout mice. Exons 5 and 6 were deleted, leading to out-of-frame products.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rasgrp4RAS guanyl releasing protein 47Rasgrp4 <tm1.2>targeted mutation 1.2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP


Donor DNAmouse RasGRP4 genomic DNA, phage P1 loxP, yeast FRT
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorToshitada Takemori (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data