Strain Data Sheet

RBRC05663

Strain Information

Image
BRC No.RBRC05663
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bcl6<tm1.1Mtto>
Former Common nameBcl6-Flox mouse, B6-Bcl6 floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2008. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6).
Strain descriptionBcl6 gene floxed mice. Exons 7 to 9 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.
Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, Kubo M, Nagashima T, Takahashi Y, Kurosaki T, Okada M, Ohara O, Rajewsky K, Takemori T
J. Exp. Med., 209, 2079-2097 (2012). 23027924

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcl6B cell leukemia/lymphoma 616Bcl6targeted mutation 1.1, Toshitada Takemori
Frtyeast FRT (flippase recombination target) site16Frt
loxPphage P1 loxP16loxP
loxPphage P1 loxP16loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal humoral immune response(MP:0001800)

  • abnormal somatic hypermutation frequency(MP:0004815)

  • absent germinal center B cells(MP:0008179)

  • decreased T follicular helper cell number(MP:0010187)

  • decreased germinal center B cell number(MP:0008178)
    • more 1 phenotypes
  • immune system phenotype(MP:0005387)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Bcl6 genome DNA
    Research applicationCre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 209, 2079-2097 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Akiko Ishige is requested.
    DepositorToshitada Takemori (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2013

    BRC mice in Publications

    Otsuka K, Yamada A, Saito M, Ushio A, Sato M, Kisoda S, Shao W, Tsunematsu T, Kudo Y, Arakaki R, Ishimaru N.
    Achaete-Scute Homologue 2-Regulated Follicular Helper T Cells Promote Autoimmunity in a Murine Model for Sjögren Syndrome.
    Am J Pathol 189(12) 2414-2427(2019) 31539517
    Koike T, Harada K, Horiuchi S, Kitamura D.
    The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets.
    Elife 8 (2019) 31225793
    Chikada H, Ida K, Nishikawa Y, Inagaki Y, Kamiya A.
    Liver-specific knockout of B cell lymphoma 6 suppresses progression of non-alcoholic steatohepatitis in mice.
    Sci Rep 10(1) 9704(2020) 32546802