Strain Data Sheet
RBRC05663
Information1 | |
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| Image | |
| BRC No. | RBRC05663 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Bcl6<tm1.1Mtto> |
| Former Common name | Bcl6-Flox mouse, B6-Bcl6 floxed |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2008. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6). |
| Strain description | Bcl6 gene floxed mice. Exons 7 to 9 were flanked by loxP sites. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | J. Exp. Med., 209, 2079-2097 (2012). 23027924 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP |
Information2 | |
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| Donor DNA | Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Bcl6 genome DNA |
| Research application | Cre/loxP system FLP/frt system Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Distinct cellular pathways select germ-line encoded and somatically mutated antibodies into immunological memory. J. Exp. Med., 209, 2079-2097 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Toshitada Takemori (RIKEN) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Mouse of the Month Jun 2013 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Otsuka K, Yamada A, Saito M, Ushio A, Sato M, Kisoda S, Shao W, Tsunematsu T, Kudo Y, Arakaki R, Ishimaru N. Achaete-Scute Homologue 2-Regulated Follicular Helper T Cells Promote Autoimmunity in a Murine Model for Sjögren Syndrome. Am J Pathol 189(12) 2414-2427(2019) 31539517 Koike T, Harada K, Horiuchi S, Kitamura D. The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. Elife 8 (2019) 31225793 Chikada H, Ida K, Nishikawa Y, Inagaki Y, Kamiya A. Liver-specific knockout of B cell lymphoma 6 suppresses progression of non-alcoholic steatohepatitis in mice. Sci Rep 10(1) 9704(2020) 32546802 |