Strain Data Sheet

RBRC05663

Information1

Image
BRC No.RBRC05663
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bcl6<tm1.1Mtto>
Former Common nameBcl6-Flox mouse, B6-Bcl6 floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2008. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6).
Strain descriptionBcl6 gene floxed mice. Exons 7 to 9 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Exp. Med., 209, 2079-2097 (2012). 23027924

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcl6B cell leukemia/lymphoma 616Bcl6<tm1.1Mtto>targeted mutation 1.1, Toshitada Takemori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Information2

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Bcl6 genome DNA
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Distinct cellular pathways select germ-line encoded and somatically mutated antibodies into immunological memory. J. Exp. Med., 209, 2079-2097 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorToshitada Takemori (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jun 2013
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Otsuka K, Yamada A, Saito M, Ushio A, Sato M, Kisoda S, Shao W, Tsunematsu T, Kudo Y, Arakaki R, Ishimaru N.
Achaete-Scute Homologue 2-Regulated Follicular Helper T Cells Promote Autoimmunity in a Murine Model for Sjögren Syndrome.
Am J Pathol 189(12) 2414-2427(2019) 31539517

Koike T, Harada K, Horiuchi S, Kitamura D.
The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets.
Elife 8 (2019) 31225793

Chikada H, Ida K, Nishikawa Y, Inagaki Y, Kamiya A.
Liver-specific knockout of B cell lymphoma 6 suppresses progression of non-alcoholic steatohepatitis in mice.
Sci Rep 10(1) 9704(2020) 32546802