Strain Data Sheet

RBRC05662

Strain Information

Image
BRC No.RBRC05662
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcp4<tm2.1(cre)>
Former Common namePcp4-cre, B6-Pcp4/Cre KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2008. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6).
Strain descriptionPcp4 cre knock-in mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site16Frt
GH Growth hormone polyA (Bovine)16GH
Pcp4
MGI:97509		Purkinje cell protein 416Pcp4 <tm2.1(cre)Mtto> targeted mutation 2.1, Toshitada Takemori
cre Phage P1 Cre recombinase16cre

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 cre recombinase, yeast FRT site, bovine growth hormone polyA signal, mouse Pcp4 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Cre driver
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Akiko Ishige is requested.
    DepositorToshitada Takemori (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Jamiolkowski RM, Nguyen QA, Farrell JS, McGinn RJ, Hartmann DA, Nirschl JJ, Sanchez MI, Buch VP, Soltesz I.
    The fasciola cinereum of the hippocampal tail as an interventional target in epilepsy.
    Nat Med 30(5) 1292-1299(2024) 38632391