RBRC05661

Information1

Image
BRC No.RBRC05661
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcp4<tm1.1>
Former Common namePcp4-flox, B6-Pcp4 floxed
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6).
Strain descriptionPcp4 floxed mice. Econ 3 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack2015/12/07Room:3-5Rack:C
2015/10/05Room:3-5Rack:C
2015/08/03Room:3-5Rack:C
2015/06/24Room:3-5Rack:C
2015/06/01Room:3-5Rack:C
2015/04/06Room:3-5Rack:C
2015/02/02Room:3-5Rack:C
2014/12/01Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcp4Purkinje cell protein 416Pcp4 <tm1.1>targeted mutation 1.1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Pcp4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Akiko Ishige is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorToshitada Takemori (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data