RBRC05660

Information1

Image
BRC No.RBRC05660
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(CAG-CAT,spH)5Yyan
Former Common nameCATV-5, B6-CAG-CAT-SpH Tg#5, B6.D2-Tg (CAG-cat, -spH) 5Yyan
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F1 fertilized eggs. The Tg mice were crossed to C57BL/6J.
Strain descriptionVesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. CAG-CAT-SpH transgenic mouse expresses SpH under the control of CAG promoter by cre-mediated recombination. This strain is useful for studies of presynaptic terminals in various regions of the brain. Tg mice mice show no obvious abnormality.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
ReferencesGenesis 42, 53-60 (2005). 15880564

Health Report

Examination Date / Room / Rack2014/04/30Room:3-DRack:C
2014/04/21Room:3-DRack:C
2014/04/21Room:3-DRack:C
2014/04/21Room:3-DRack:C
2014/02/18Room:3-DRack:C
2013/12/16Room:3-DRack:C
2013/10/21Room:3-DRack:C
2013/09/02Room:3-DRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vamp2vesicle-associated membrane protein 2UNVamp2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
pHluorinpH-sensitive green fluorescent proteins, S147E, N149L, I161T, V163A, N164I, K166Q, I167V, R168H, S202H and L231H. (Jellyfish)UNpHluorin

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli Chloramphenicol acetyltransferase (CAT), SV40 Small T antigen polyA, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis 42, 53-60 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOURCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University.
DepositorHiromu Yawo (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data