BRC No.RBRC05658
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Siglech<tm1.1Ksat>
Former Common nameSiglech<dtr>, B6-Siglech/DT-GFP KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Strain developmentDeveloped by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. The mutant mice were backcross to C57BL/6J.
Strain descriptionIRES-DTR-EGFP cassette were inserted in to 3’ untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene. Plasmacytoid dendritic cells (pDCs) from homozygous mutant mice are defect in the transcriptional and cell surface expression of Siglech. In mice with diphtheria toxin (DT) induced specific ablation of pDCs.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesImmunity, 35, 958-971 (2011). 22177923
J. Allergy Clin. Immunol., (2018). 29477579

Health Report

Examination Date / Room / Rack2013/07/08Room:2-検Rack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Siglechsialic acid binding Ig-like lectin H7Siglech<tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)7EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBEGFheparin-binding EGF-like growth factor (human)7HBEGF

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP


Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP, Phage P1 loxP site, mouse Siglech genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes" specified by the DEPOSITOR and Inserm.
DepositorKatsuaki Sato (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
MTA for Siglech-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Depositor's present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.

BRC mice in Publications

No Data