Strain Data Sheet



BRC No.RBRC05654
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-mRFP1,-SOX9,EGFP)1Haak
Former Common nameB6-CAG-SOX9 Tg
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Haruhiko Akiyama, Kyoto University Hospital in 2007. The mice were crossed to C57BL/6.
Strain descriptionSox9, SRY-box containing gene 9 conditional transgenic mice. The transgenes consists of CAG promoter and a floxed monomeric red fluorescence protein 1 (mRFP1) cassette and Sox9-EGFP. Cre expression removes the mRFP1 gene, allowing expression of Sox9 and EGFP protein.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]
ReferencesJ. Bone Miner. Metab., 29, 123-129 (2011). 20676705

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SOX9SRY-box containing gene 9 (human)UNSOX9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tg(CAG-mRFP1,-SOX9,-EGFP)1HaakEnhanced Green Fluorescent Protein (Aequorea victoria)UNTg(CAG-mRFP1,-SOX9,-EGFP)1Haaktransgene insertion 1, Haruhiko Akiyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mRFP1monomeric Red Fluorescent Protein (Discosoma sp.)UNmRFP1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)


Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Entacmaea quadricolor mRFP1 cDNA, bovine four tandem polyadenylation sites, phage P1 loxP sites, Human influenza virus Ha(hemagglutinin) tag, human Sox9 cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Aequorea victoria EGFP cDNA, bovine polyA signal
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The BIOLOGICAL RESOURCE shall be distributed solely to non-profit organizations for academic research purposes to publish the research results. The BIOLOGICAL RESOURCE must not be used in any for-profit organizations. (2) The RECIPIENT shall obtain prior writen consent to use the BIOLOGICAL RESOURCE from the DEPOSITOR. The consent may be obtainted through internet. (3) After obtaining the consent from the DPOSITOR, the RECIPIENT shall execute the MTA for the use of mRFP 1.0 with Dr. Roger Tsien, UCSD (University of California, San Diego) and send the PDF file of the executed MTA to RIKEN BRC. The form of the MTA may be downloaded at the website of Dr. Tsuen’s laboratory ( (4) The RECIPIENT agrees to provide appropriate acknowledgements to the DEPOSITOR and Dr. Roger Tsien in all publications. The RECIPIENT also agrees to cite “J Bone Miner Metab. 2011 29(1):123-9” in all publications. (5) In the event that an invention is derived from the research using BIOLOGICAL RESROUCE, Recipient shall notify the Depositor upon filing a patent application claiming MODIFICATIONS or method(s) of manufacture or use(s) of the BIOLOGICAL RESROUCE. (6) RIKEN BRC shall send the copy of the Material Transfer Agreement execututed between the RECIPIENT and RIKEN BRC to UCSD.
(7) The RECIPIENT agrees to the following terms and condions requested by HHMI(Howard Hughes Medical Institute) and UCSD:The material being transferred to you contains material received from Dr. Roger Tsien, an investigator of the Howard Hughes Medical Institute at the University of California, San Diego (“Tsien material”). The Tsien material is experimental in nature and must be used with prudence and appropriate caution, since not all of its characteristics are known. THE TSIEN MATERIAL IS PROVIDED WITHOUT WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE OR ANY OTHER WARRANTY, EXPRESS OR IMPLIED. THE TSIEN MATERIAL IS PROVIDED WITHOUT REPRESENTATION OR WARRANTY THAT THE USE OF THE TSIEN MATERIAL WILL NOT INFRINGE ANY PATENT OR OTHER PROPRIETARY RIGHT. It cannot be used for any commercial purpose or for work on human subjects, including diagnostic testing. Should the use of this material result in one or more scientific publication(s) you should acknowledge in the paper(s) that the material contained material provided by Dr. Roger Tsien.
The Tsien material must not be distributed to laboratories of for-profit companies. The Tsien material may be distributed to other non-profit laboratories (a) with the prior consent of Dr. Roger Tsien, (b) after an agreement is executed that contains terms substantially similar to the terms set forth herein, including prohibition against further transfers of the Tsien material and use of the Tsien material for commercial purposes or for work on human human subjects, including diagnostic testing, and (c) written notice of the transfer is sent to University of California, San Diego, Technology Transfer and Intellectual Property Services, 9500 Gilman Drive, #0910, La Jolla, CA 92093-0910, Attention: Assistant Vice Chancellor-IP. Written notice may also be emailed to, or faxed to 858-534-7345.
DepositorHaruhiko Akiyama (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

Arai HN, Sato F, Yamamoto T, Woltjen K, Kiyonari H, Yoshimoto Y, Shukunami C, Akiyama H, Kist R, Sehara-Fujisawa A.
Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate.
Cell Rep 29(3) 603-616.e5(2019) 31618630