Strain Information | |
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Image | |
BRC No. | RBRC05651 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Sox9<tm1Haak> |
Former Common name | Sox9-EGFP KI, Sox9-EGFP knock-in, B6-Sox9/GFP KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. A loxP-flanked PGK-neo-bpA was removed by cre-mediated recombination. C57BL/6 and 129 mixed background. |
Strain description | Sox9-EGFP knock-in mice. An IRES-EGFP-pA cassette was inserted into the 3’ untranslated region in exon 3 of Sox9 gene via homologous recombination. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] |
References | Morphometric analysis of testis cord formation in Sox9-EGFP mice. Nel-Themaat L, Vadakkan T J, Wang Y, Dickinson M E, Akiyama H, Behringer R R Dev. Dyn., 238, 1100-1110 (2009). 19384968 |
Health Report | |
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Examination Date / Room / Rack | 2024/06/10Room:3-3Rack:C 2024/03/11Room:3-3Rack:C 2023/12/11Room:3-3Rack:C 2023/09/11Room:3-3Rack:C |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 11 | EGFP | ||||
IRES | internal ribosomal entry site (EMCV) | 11 | |||||
Sox9 | SRY (sex determining region Y)-box 9 | 11 | Sox9 | targeted mutation 1, Haruhiko Akiyama | |||
loxP | phage P1 loxP | 11 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | EMCV internal ribosomal entry site (ires), jellyfish EGFP cDNA, phage P1 loxP site, bovine polyA signal, mouse Sox9 genomic DNA[update: Dec. 12, 2013] |
Research application | Cre/loxP system Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 238, 1100-1110 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient’s use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes. |
Depositor | Benoit de Crombrugghe (The University of Texas M. D. Anderson Cancer Center) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |