RBRC05650

Information1

Image
BRC No.RBRC05650
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Zrsr1<tm1Mok>
Former Common nameUNG, B6;129S-Zrsr1<tm1Mok>, u2af1-rs1 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionu2af1-rs1 (Zrsr1) gene knockout mice. The entire coding region and about 3/4 of the CpG islands including region II were replaced with a PGK-gpt-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 268, 590-595 (2000). 10679248

Health Report

Examination Date / Room / Rack2013/07/08Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Zrsr1zinc finger (CCCH type), RNA binding motif and serine/arginine rich 111Zrsr1<tm1Mok>targeted mutation 1, Motoya Katsuki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli xanthine phosphoribosyltransferase (Ecogpt), E. coli neomycin resistance gene, mouse u2af1-rs1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 268, 590-595 (2000).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshikuni Sasaoka (Niigata University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data