Strain Data Sheet

RBRC05649

Information1

Image
BRC No.RBRC05649
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Plin5<tm1Tosu>/Rbrc
Former Common nameB6-Plin5 KO, B6.129S4-Plin5<tm1>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tomohiro Yamaguchi and Takashi Osumi, Graduate School of Life Science, University of Hyogo in 2007. J1 ES cells derived from 129S4/SvJae were used. The mutant mice were backcrossed to C57BL/6JJcl.
Strain descriptionPlin5 perilipin 5 gene knockout mice. Exons 2 to 4 were replaced with a floxed PGK-neo cassette. In homozygous mutant mice, heart lipid droplets were absent and the activity of fatty acid oxidation was increased.
Colony maintenance
ReferencesJ. Biol. Chem., 281, 14232-14240 (2006). 16571721
J. Biol. Chem., 287, 23852-23863 (2012). 22532565
Mol. Cell. Biol., (2014). 24820416

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Plin5perilipin 517Plin5<tm1Tosu>targeted mutation 1, Takashi Osumi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)17GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNABacteriophage P1 loxP sites, E. coli Neomycin resistance gene, Bovine Growth hormone gene polyadenylation site, Mouse Phosphoglycerate kinase (PGK) gene promoter, mouse perilipin5 genomic DNA
Research applicationCardiovascular Research
Cre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 287, 23852-23863 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTakashi Osumi (University of Hyogo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data