RBRC05646

Information1

Image
BRC No.RBRC05646
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namePLCeta(Acc.No.CDB0463K)
Former Common nameB6-Plch2 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Fukami Kiyoko, Tokyo University of Pharmacy and Life Sciences.
Strain descriptionPLCeta2 (Plch2) gene knockout mice. The first to third exons of PLCeta2 gene were replaced with a loxP/LacZ/frt-flanked neo/loxP/pA cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesGene Expr. Patterns, 10, 119-126 (2010). 20060933

Health Report

Examination Date / Room / Rack2013/06/17Room:3-CRack:G
2013/04/15Room:3-CRack:G
2013/02/18Room:3-CRack:G
2012/12/10Room:3-CRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Plch2phospholipase C, eta 24Plch2<tm1Kfu >targeted mutation 1, Kiyoko Fukami

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence4lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPLceta KO マウス (LacZ, Neo 耐性遺伝子 : 大腸菌由来 / loxP, lox71 : P1ファージ由来 / frt : 酵母由来 / rabbit beta, globin 3' end : ウサギ)
Research application
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the Dr.Kiyoko Fukami (E-mail: kfukami@toyaku.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0463K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. Gene Expr Patterns. 2010 10(2-3):119-26. (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data